Heterologous expression of SARS-CoV ORF10 and X5 genes in E. coli and Streptomyces lividans TK24.
Identifieur interne : 006740 ( Main/Exploration ); précédent : 006739; suivant : 006741Heterologous expression of SARS-CoV ORF10 and X5 genes in E. coli and Streptomyces lividans TK24.
Auteurs : Jiang-Qiang Kong [République populaire de Chine] ; Wei Wang ; Guan-Hua Du ; Ping Zhu ; Ke-Di ChengSource :
- Zeitschrift fur Naturforschung. C, Journal of biosciences [ 0939-5075 ]
Descripteurs français
- KwdFr :
- ADN viral (génétique), ADN viral (isolement et purification), Cadres ouverts de lecture, Escherichia coli (génétique), Gènes viraux, Protéines recombinantes (isolement et purification), Protéines virales (génétique), Protéines virales (isolement et purification), Streptomyces lividans (génétique), Vecteurs génétiques, Virus du SRAS (génétique).
- MESH :
- génétique : ADN viral, Escherichia coli, Protéines virales, Streptomyces lividans, Virus du SRAS.
- isolement et purification : ADN viral, Protéines recombinantes, Protéines virales.
- Cadres ouverts de lecture, Gènes viraux, Vecteurs génétiques.
English descriptors
- KwdEn :
- DNA, Viral (genetics), DNA, Viral (isolation & purification), Escherichia coli (genetics), Genes, Viral, Genetic Vectors, Open Reading Frames, Recombinant Proteins (isolation & purification), SARS Virus (genetics), Streptomyces lividans (genetics), Viral Proteins (genetics), Viral Proteins (isolation & purification).
- MESH :
- chemical , genetics : DNA, Viral, Viral Proteins.
- chemical , isolation & purification : DNA, Viral, Recombinant Proteins, Viral Proteins.
- genetics : Escherichia coli, SARS Virus, Streptomyces lividans.
- Genes, Viral, Genetic Vectors, Open Reading Frames.
Abstract
In previous studies a variety of novel accessory genes has been identified that were interspersed among the structural genes of the SARS-CoV (severe acute respiratory syndrome coronavirus) genome. The predicted unknown proteins (PUPs) encoded by the accessory genes, which are considered to be unique to the SARS-CoV genome, might play important roles in the SARS-CoV infection. Two of these genes, called ORF10 and X5, were synthesized and introduced into E. coli and Streptomyces lividans TK24, respectively. SDS-PAGE and Western blot revealed that the ORF10 and X5 genes have been expressed in the two hosts. This is the first report of heterologous expression of ORF10 and X5 genes in E. coli and S. lividans TK24. This work makes it possible to study the structure and potential functions of proteins encoding by these two genes.
DOI: 10.1515/znc-2007-9-1021
PubMed: 18069252
Affiliations:
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Le document en format XML
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The predicted unknown proteins (PUPs) encoded by the accessory genes, which are considered to be unique to the SARS-CoV genome, might play important roles in the SARS-CoV infection. Two of these genes, called ORF10 and X5, were synthesized and introduced into E. coli and Streptomyces lividans TK24, respectively. SDS-PAGE and Western blot revealed that the ORF10 and X5 genes have been expressed in the two hosts. This is the first report of heterologous expression of ORF10 and X5 genes in E. coli and S. lividans TK24. This work makes it possible to study the structure and potential functions of proteins encoding by these two genes.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Cheng, Ke Di" sort="Cheng, Ke Di" uniqKey="Cheng K" first="Ke-Di" last="Cheng">Ke-Di Cheng</name><name sortKey="Du, Guan Hua" sort="Du, Guan Hua" uniqKey="Du G" first="Guan-Hua" last="Du">Guan-Hua Du</name><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name><name sortKey="Zhu, Ping" sort="Zhu, Ping" uniqKey="Zhu P" first="Ping" last="Zhu">Ping Zhu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Kong, Jiang Qiang" sort="Kong, Jiang Qiang" uniqKey="Kong J" first="Jiang-Qiang" last="Kong">Jiang-Qiang Kong</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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